期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 166, 期 -, 页码 977-985出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.10.254
关键词
Sulfated polysaccharide; Eucheuma serra; Fond allergy; Intestinal microbiota; Ovalbumin
资金
- National Key Research and Development Program of China [2019YFC1605003-3]
- Science and Technology Projects of Xiamen Science and Technology Bureau [3502Z20183034]
DESP can improve food allergy by regulating the intestinal microbiota, suppressing allergy-related cytokines and symptoms, reducing intestinal inflammation.
The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work. Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-alpha) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice. After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed. Moreover, the reduced intestinal inflammation was discovered in the DESP-treated group. Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and alpha-diversity analysis revealed that DESP improved microbial community richness. Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bucteroidetes/Proteobucteriu. Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae). These findings, therefore, suggest that DESP may ameliorate food allergy through the regulation of intestinal microbiota. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据