Physicochemical characterization and antioxidant effects of green microalga Chlorella pyrenoidosa polysaccharide by regulation of microRNAs and gut microbiota in Caenorhabditis elegans

A novel polysaccharide from Chlorella pyrenoidosa (CPP) was separated and purified with the average molecular weight 15.8 kDa. It was composed of seven monosaccharides including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. FT-IR and NMR spectra analysis further revealed that CPP was an acidic polysaccharide consisting of beta-L-Arap-(1 ->,-> 2)-alpha-L-Rhap-(1 ->, beta-D-GlcpA-(1 ->,-> 4)-alpha-D-GalpA(1 ->,-> 6)-beta-D-Glcp-(1 ->,-> 3)-beta-D-Manp-(1 ->, and -> 3, 6)-beta-D-Galp-(1 ->. The CPP treatment could effectively prolong lifespan of Caenorhabditis elegans under the oxidative stress conditions and inhibit the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) as well as enhancing the level of superoxide dismutase (SOD). It could up-regulate the expressions of Daf-16 and Skn-1 genes via declining miR-48-3p, miR-48-5p, and miR-51-5p translocation. Moreover, 16S rRNA sequencing revealed that the CPP-enriched Faecalibacterium, Haemophilus, Vibrio, and Shewanella were strongly correlated with SOD, MDA, apoptosis, and ROS. These results indicated that CPP may be considered as a desired ingredient on regulating the aging and oxidative diseases. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The novel polysaccharide CPP from Chlorella pyrenoidosa showed potential in prolonging lifespan, inhibiting oxidative stress, and regulating gene expression in Caenorhabditis elegans. The study suggested that CPP could be considered as a desirable ingredient for regulating aging and oxidative diseases.