期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 164, 期 -, 页码 2861-2872出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.072
关键词
Trichosanthes kirilowii Maxim seeds; Polysaccharides; Immunomodulatory activity; Microwave-assisted extraction
资金
- Jilin Province Development and Reform Commission [2019C045-4]
- Department of Science and Technology of Jilin Province [20190304102YY]
Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD). The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 degrees C, microwave power 570 W, extraction time 26 min. Under this conditions, the mean value of TKMSP yield 2.43 +/- 0.45% (n = 3), which was consistent closely with the predicted value (2.44%). The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52. TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc. However, there was no Fuc in TKMSP-3, while TKMSP-5 lacked GluA, GalA and Fuc. UV-vis and FT-IR analysis combinedwithmolecularweight determination further indicated that the five fractionswere polydisperse polysaccharides. A significant difference was achieved in the structural characterization of these five fractions. TKMSP exhibited immunosuppressive activity on RAW264.7 cells. It can be applied as a potential immunosuppressant agent in medicine. (C) 2020 Elsevier B.V. All rights reserved.
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