期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 167, 期 -, 页码 845-853出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.11.040
关键词
Longan polysaccharide; Structural characteristics; Immunomodulatory activity; Ca2+/CR3 receptor
资金
- Earmarked Fund for China Agriculture Research System [CARS-32]
- Yang Fan Innovative and Entrepreneurial Research Team Project [2014YT02H013]
- International Training for Outstanding Scientific Talents Program from South China Agricultural University [2018YQGP-BS007]
The water-soluble glucose-rich polysaccharide LPsx from dried 'Shixia' longan pulp has been isolated and studied for its structure and immuno-regulatory mechanism. LPsx showed immunomodulatory activity by enhancing macrophage phagocytosis and promoting the production of various inflammatory factors, while also inhibiting the inflammatory response induced by lipopolysaccharide. The mechanism of immuno-regulation was found to involve Ca2+ and CR3-mediated MAPKs and PI3K-AKT signaling pathways in macrophage activation.
Awater-soluble glucose-rich polysaccharide from dried 'Shixia' longan pulp (LPsx) has been isolated for the first time, and its structure and immuno-regulatory mechanism were studied. LPsx is a hetero-polysaccharide with the average molecular weight 4102 g/mol. It was mainly consisted of glucose (95.9%), and small proportions of arabinose (2.1%), galactose (1.0%), mannose (0.6%), and xylose (0.4%). As analyzed by NMR, LPsx was mainly composed of (1 -> 6)-alpha-D-glucose and (1 -> 6)-beta-D-glucose, branched with alpha-D-glucose-(1 ->. The immunomodulatory activity study showed that LPsx significantly increased the phagocytosis of macrophages, and strongly promoted the production of NO, IL-1 beta, IL-6 and TNF-alpha. Moreover, LPsx could inhibit the inflammatory response induced by lipopolysaccharide. The immuno-regulatorymechanism of LPsx was studied using RNA- sequencing and receptors activity analyses. It was found that LPsx induced macrophage activation via Ca2+ and CR3-mediated MAPKs and PI3K-AKT signaling pathways. The results would be helpful for revealing the health promoting mechanism of dried 'Shixia' longan in traditional Chinese medicine. (C) 2020 Elsevier B.V. All rights reserved.
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