Lignin from oil palm empty fruit bunches: Characterization, biological activities and application in green synthesis of silver nanoparticles

Lignin was extracted from oil palm empty fruit bunches under four different conditions. The lignin samples were characterized and employed in the green synthesis of silver nanoparticles. Two-dimensional HSQC NMR analysis showed that lignins extracted under more aggressive conditions (3.5% acid, 60 min) exhibited less signals and thus, presented a more degraded chemical structure. Additionally, those lignins obtained under harsh conditions (3.5% acid, 60 min) exhibited higher antioxidant capacity than those obtained under mild conditions (1.5% acid, 20 min). Formation of lignin-mediated silver nanoparticles was confirmed by color change during their synthesis. The surface plasmon resonance peaks (423-427 nm) in UV-visible spectra also confirmed the synthesis of AgNPs. AgNPs showed spherical shape, polycrystalline nature and average size between 18 and 20 nm. AgNPs, in suspension, presented a negative Zeta potential profile. Lignin was assumed to contribute in the antioxidant capacity exhibited by AgNPs. All AgNPs presented no significant differences on the disk diffusion antimicrobial susceptibility test against E. coli. The minimum inhibitory concentration of HAL3-L AgNPs (62.5 mu g.mL(-1)) was better than other physicochemically produced AgNPs (100 mu g.mL(-1)). (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

Extraction conditions of lignin affect its chemical structure and antioxidant capacity, with lignin playing a role in the antioxidant activity of silver nanoparticles. Synthesized silver nanoparticles exhibit good antimicrobial properties, with HAL3-L AgNPs showing better antibacterial effects.