期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 165, 期 -, 页码 1694-1705出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.10.029
关键词
Radix Puerariae lobatae; Polysaccharide; Food function; Hyperlipidemia; Bile acid metabolism; FXR signaling pathway
资金
- National Key Research and Development Program of China [2017YFC1702902, 2019YFC1712300]
- Jiangxi university of traditional Chinese medicine 1050 youth talent project, Double Hundred Plan for high-level scientific and Technological Innovation Talents in Nanchang(China)
Polysaccharides are important active constituents of Radix Puerariae lobatae (RPL). In this study, a novel homogeneous polysaccharide from RPL was successfully obtained by HP-20 macroporous resin and purified by Sepharose G-100 column chromatography. Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of alpha-1,3-linked and alpha-1,4-linked glucose. The molecular weight of PL-S2 was 18.73 kDa. The hypolipidemic effect of PL-S2 on hyperlipidemic rats was evaluated in histopathology and metabolomics analyses. PL-S2 significantly reduced plasma lipid levels and inhibited bile acid metabolism. We also demonstrated that treatment with PL-S2 activated FXR, CYP7A1, BESP, and MRP2 in rat liver. Our findings first indicate that PL-S2 decreases plasma lipid levels in hyperlipidemic rats by activating the FXR signaling pathway and promoting bile acid excretion. Therefore, PL-S2 derived from RPL is implicated as a functional food factor with lipid-regulating activity, and highlighted as a potential food supplement for the treatment of hyperlipidemia. (C) 2020 Elsevier B.V. All rights reserved.
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