期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 164, 期 -, 页码 3953-3964出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.09.006
关键词
Carboxymethyl chitosan; Marine collagen peptide; Optimization; Coagulation
资金
- Natural Science Foundation of Guangdong Province of China [2016A030308009]
- Project of Science and Technology Plan of Zhanjiang [2019A01017]
- Project of Enhancing School with Innovation of Guangdong Ocean University [2017KTSCX090]
- Graduate Education Innovation Program of Guangdong Ocean University [201926]
Uncontrolled bleeding has always been a sudden accident, which is the main cause of casualties in war trauma, emergency events and surgical operations. Rapid hemostatic materials can effectively reduce casualties and save lives. In this paper, marine collagen peptide grafted carboxymethyl chitosan (CMCS-MCP) was synthesized by 1ethyl-(dimethylaminopropyl) carbodiimide (EDC)-mediated coupling reaction. To obtain CMCS-MCP conjugates with different degrees of substitution (DS), the reaction conditions were investigated by single-factor tests and optimized by response surface methodology. And the sponges of CMCS-MCP were prepared by freeze-thaw cycling and freeze-drying and characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), and X-ray diffraction (XRD). To evaluate the hemostatic properties of CMCS-MCP sponges, in vitro and in vivo hemostasis tests were carried out. The results showed that the optimum preparation conditions were the mass ratio of MCP to CMCS (MMCP/MCMCS) 6:1, reaction temperature 41 degrees C, and reaction time 16 h. And under which the DS of 58.86% was obtained. Structure analysis showed that MCP had been successfully grafted onto the CMCS molecular chain, and the CMCS-MCP sponges were of high porosity. In vitro and in vivo hemostasis tests showed that the CMCS-MCP sponges had significant procoagulant activities, especially the one with high DS of 58.86%. The hemostasis mechanism may be that the synergistic effects of MCP and CMCS accelerated coagulation through multiple approaches. The CMCS-MCP sponges give a new insight into biomedical hemostasis materials. (C) 2020 Elsevier B.V. All rights reserved.
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