期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 164, 期 -, 页码 949-962出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.111
关键词
Sebastes schlegelii; Genome-wide; Toll-like receptor; Bacterial infection; Expression profiles
资金
- Young Experts of Taishan Scholars [tsqn201909130]
- Science and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province [2019KJF003]
- First Class Fishery Discipline Programme in Shandong Province, China
- special talent programme One Thing One Decision (Yishi Yiyi) Programme in Shandong Province, China
As one of the key components of pattern recognition receptors, Toll-like receptors (TLRs) play pivotal roles in the innate immune system. However, little information is available about the TLR genes in Sebastes schlegelii. In the present study, 17 TLR genes were identified and classified based on the whole genome database. Tandem duplication events in TLR1, TLR2, TLR5 and TLR13 played major role in the expansion of S. schlegelii TLR genes; both TLR2-3 and TLR2-4 had the same largest number of introns/exons, 11 exons and 10 introns. The syntenic analysis showed neighboring genes of TLR genes were most conserved in S. schlegelii and in L. crocea. Phylogenetic and evolutionary analysis showed that these TLR genes were divided into five subfamilies and exhibited different selection pressures. Meanwhile, the expression patterns of TLR genes in the intestine after E. tarda infection were investigated by qRT-PCR. Finally, protein and protein interaction (PPI) network analysis indicated that TLR genes interacted with IFN-inducible genes, inflammatory cytokines, and participated in MyD88-dependent pathway. In summary, this study provided valuable information for further functional characterization of TLR genes in S. schlegelii. (C) 2020 Elsevier B.V. All rights reserved.
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