期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 164, 期 -, 页码 3687-3697出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.206
关键词
Tamarindus indica L. pulp; Arabinoglucan; Structural analysis
资金
- National Key Research and Development Program of China [2018YFC1604405]
- National Natural Science Foundation of China [31871753]
- Science and Technology Commission of Shanghai Municipality, China [19DZ2284200]
In this study, an ultrasonic-extracted polysaccharide (nCPTP-55) was obtained with the highest yield (61.08%, w/w) from tamarind pulp, which consisted chiefly of total sugar (85.98%, w/w) with few protein (2.10%, w/w). Monosaccharide analysis showed nCPTP-55 was mainly composed of arabinose (39.19 mol%) and glucose (50.48 mol%) with negligible GlcA (2.05 mol%), indicating the neutral nature of nCPTP-55, which was further elucidated structurally via GC-MS and NMR, i.e., an arabinoglucan composed of -> 3)-beta-D-Glcp-(1 -> backbone with only T-alpha-L-Araf-(1 -> branched at O-4 (27.82%) and O-6 (39.99%), resulting in relatively high A/G ratio (0.68-0.70). Based on MM2 minimized energy, the 3D schematic structures of nCPTP-55 could be considered as structural basis for its conformational behavior, which was preliminarily estimated via HPSEC-MALLS as between compact sphere and loosely hyper-branched chain (rho = 0.84). Therefore, the relationship between molecular structure and conformational behavior was basically established for nCPTP-55, which was in a bid to have a better knowledge of its structure-property and structure-bioactivity relationships potentially required for more applications in food, cosmetic and pharmaceutical fields. (C) 2020 Elsevier B.V. All rights reserved.
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