Antibacterial properties and cytocompatibility of biobased nanofibers of fish scale gelatine, modified polylactide, and freshwater clam shell

We report herein new nanofibers prepared from fish scale gelatine (FSG), modified polylactide (MPLA), and a natural antibacterial agent of freshwater clam(Corbicula fluminea Estefania) shell powder (FCSP). A preparation of FSG fromMullet scales is also described. To improve the biocompatibility and antibacterial activity of the nonwoven nanofibers, MPLA/FCSP was added to enhance their antibacterial properties. FSG was then combined with MPLA/FCSP using an electrospinning technique to improve the biocompatibility of the as-fabricated 100-500 nm-diameter non-wovenMPLA/FCSP/FSG nanofibers. The resulting tensile properties andmorphological characteristics indicated enhanced adhesion among FSG, FCSP, and MPLA in the MPLA/FCSP/FSG nanofibers, as well as improved water resistance and tensile strength, compared with the PLA/FSG nanofibers. MTT assay, cell-cycle, and apoptosis analyses showed that both PLA/FSG and MPLA/FCSP/FSG nanofibers had good biocompatibility. Increasing the FSG content in PLA/FSG andMPLA/FCSP/FSG nanofibers enhanced cell proliferation and free-radical scavenging ability, but did not affect cell viability. Quantitative analysis of bacteria inhibition revealed that FCSP imparts antibacterial activity. (C) 2020 Elsevier B.V. All rights reserved.