期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 165, 期 -, 页码 1055-1065出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.09.167
关键词
Nanoemulsion; Piplartine; Topical; Skin cancer; Alginate; Chitosan
资金
- Sao Paulo Research Foundation (FAPESP) [2018/13877-1, 2015/17177-6]
- National Council of Technological and Scientific Development (CNPq) [443549/2014-1]
- CAPES [001]
- FAPESP [2016/16554-3, 2017/07856-9, 2017/04174-4, 2018/18813-1, 2020/01208-8]
- SisGen [A90EE5B]
- CNPq
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [18/13877-1, 15/17177-6, 17/07856-9] Funding Source: FAPESP
Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine delivery and formulation efficacy were evaluated. The nanoemulsion droplets displayed similar size (96-112 nm), but opposite charge; the polysaccharides improved piplartine penetration into and across the skin (1.3-1.9-fold) in a similar manner, increasing the ratio drug in the skin/receptor phase by 1.4-1.5-fold compared to the plain NE and highlighting their relevance for cutaneous localization. Oleic acid addition to the chitosan-containing NE further increased drug penetration (similar to 1.9-2.0-fold), as did increases in drug content from 0.5 to 1%. The cytotoxicity of piplartine was similar to 2.8-fold higher when the drug was incorporated in the chitosan-containing NE compared to its solution (IC50 = 14.6 mu M) against melanoma cells. The effects of this nanocarrier on 3D melanoma tissues were concentration-related; at 1%, piplartine elicited marked epidermis destruction. These results support the potential applicability of the chitosan-modified nanoemulsion containing piplartine as a new strategy for local management of skin cancer. (C) 2020 Elsevier B.V. All rights reserved.
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