期刊
INFLAMMATION
卷 44, 期 2, 页码 780-794出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-020-01377-9
关键词
VVN001; inflammation; endotoxin-induced uveitis; NF-kappa B signaling pathway; NLRP3 inflammasome
资金
- National Natural Science Foundation of China [81770949]
- Henan Key Laboratory of Ophthalmology and Vision Science [CKQ20180099]
VVN001 pretreatment effectively reduced inflammatory responses in human RPE cells and in a mouse model of EIU by inhibiting the overproduction of ICAM-1 and blocking the activation of the NLRP3 inflammasome and NF-kappa B signaling pathway.
To investigate protective effects of VVN001 on lipopolysaccharide (LPS)-induced inflammatory response in human retinal pigment epithelial (RPE) cells and in a mouse model of endotoxin-induced uveitis (EIU), and to explore the underlying mechanisms. Human primary RPE (hRPE) and ARPE-19 cells were pretreated with or without VVN001 for 1 h followed by 10 mu g/mL LPS stimulation for 24 h. mRNA, and protein levels of inflammatory cytokines were analyzed with real-time PCR, western blotting, and ELISA. EIU was induced by intravitreal injection of 125 ng LPS in female BALB/c mice. VVN001 eye drops (1%) were locally administrated every 4 h for 24 h after LPS injection. Clinical scores were assessed with a slit lamp. mRNA and protein levels of inflammatory cytokines were investigated simultaneously. Compared with the LPS group, VVN001 pretreatment significantly reduced mRNA expressions of intercellular adhesion molecule-1 (ICAM-1), IL-6, IL-8, TNF-alpha, IL-1 beta, IL-18, caspase-1 in hRPE, and ARPE-19 cells. Protein overproduction of ICAM-1, TNF-alpha, IL-1 beta, NLRP3, caspase-1 P20, and p-I kappa B alpha/I kappa B alpha stimulated by LPS was suppressed by VVN001 pretreatment. In vivo, VVN001 significantly reduced the average clinical score from 5.0 to 1.3 in EIU mice. Furthermore, overproduction of ICAM-1, IL-1 beta, NLRP3, caspase-1 P20, and p-I kappa B alpha/I kappa B alpha at mRNA and protein levels were remarkably suppressed by VVN001. VVN001 alleviated the inflammatory response induced by LPS both in vitro and in vivo. The effect of anti-inflammation is associated with inhibiting the overproduction of ICAM-1 and blocking the activation of NLRP3 inflammasome and the NF-kappa B signaling pathway.
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