期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
卷 57, 期 3, 页码 493-498出版社
SPRINGER
DOI: 10.1007/s11627-020-10146-0
关键词
Regeneration; In vitro propagation; Conservation; Organogenesis
资金
- World Bank
- National Agricultural Research Organisation (NARO), Uganda
The African ginger is an endangered medicinal plant facing over-utilization and destruction in the wild. This study developed an efficient in vitro propagation method, providing a route for mass production and conservation of the plant.
The African ginger (Mondia whitei) is an endangered, endemic African medicinal plant, widely used as an aphrodisiac and to manage gastrointestinal problems and anorexia. Due to high demand, it is over-utilized from the wild without replenishment and destroyed by inappropriate harvesting methods. Therefore, there is a need to develop an efficient conservation system for the African ginger. This study focused on developing an in vitro propagation procedure for the African ginger through indirect shoot organogenesis. Optimal callus formation was induced from leaf explants cultured on Murashige and Skoog (MS) basal medium fortified with 1.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D). The 1.5 mg L-1 6-benzylaminopurine (BAP) concentration was optimal with 50% of the callus-forming buds. For shoot formation, media with 2.0 mg L-1 BAP and 1.0 mg L-1 NAA resulted in 60% of the buds developing shoots. Regenerated shoots were rooted on woody plant medium (WPM) fortified with 0.1 mg L-1 BAP and no rooting hormone included. The survival rate of weaned and acclimatized plants was 70% and up to 80% respectively. The study demonstrates an in vitro propagation route for the African ginger which could support its mass production for medicinal use, availing of planting materials, conservation, and commercialization purposes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据