期刊
GYNECOLOGICAL ENDOCRINOLOGY
卷 37, 期 3, 页码 269-272出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09513590.2021.1876653
关键词
Bazedoxifene; endothelium; proliferation; cyclins
资金
- Consumers, Health, Agriculture and Food Executive Agency (CHAFEA) of the European Commission, under the European Union Health Programme (2014-2020) [664367]
The study revealed that Bazedoxifene has a proliferative effect similar to estradiol in cultured human umbilical artery endothelial cells, while the combination of Bazedoxifene and estradiol did not cause significant changes. Different compounds showed varying effects on cell proliferation at the expression level.
Objective Endothelial dysfunction and denudation are considered a first step in atherosclerosis. Endothelial proliferation is key for cellular repair. The effect of bazedoxifene on the vascular endothelium has not been explored. We investigated the effect of bazedoxifene on endothelial cell proliferation. Methods Primary cultures from human umbilical artery endothelial cells were used in dose-response experiments (0.1, 1.0, and 10.0 EC50 dose) with bazedoxifene, estradiol, raloxifene and a combination of bazedoxifene and estradiol. Proliferation was assessed with the XTT colorimetric cell-proliferation assay. The possible participation of cyclins A, B, D1 and p27(Kip1) was analyzed by the measurement of their expression at both the protein and the gene levels. Results A significant increase of similar size for cell proliferation was obtained with bazedoxifene, estradiol and raloxifene, but no significant change was observed for the association of bazedoxifene and estradiol. The impact was detected at the first 0.1 EC50 dose and was not dose-dependent. Estradiol achieved a significant increase in the protein expression of cyclin A and p27(Kip1), but no change was detected for the other compounds at either the gene or protein level. Conclusion Bazedoxifene demonstrated a proliferative effect of similar size to estradiol in cultured human umbilical artery endothelial cells. The molecular mechanisms need further investigation.
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