Long noncoding RNA expression profiles in intermittent parathyroid hormone induced cementogenesis

The aim of this study was to explore the involvement of long noncoding RNAs (lncRNAs) during intermittent parathyroid hormone (PTH) induced cementogenesis. Expression profiles of lncRNAs and mRNAs were obtained using high-throughput microarray. Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and coding-noncoding gene coexpression networks construction were performed. We identified 190 lncRNAs and 135 mRNAs that were differentially expressed during intermittent PTH-induced cementogenesis. In this process, the Wnt signaling pathway was negatively regulated, and eight lncRNAs were identified as possible core regulators of Wnt signaling. Based on the results of microarrray analysis, we further verified the repressed expression of Wnt signaling crucial components beta-catenin, APC and Axin2. Above all, we speculated that lncRNAs may play important roles in PTH-induced cementogenesis via the negative regulation of Wnt pathway.

Automated summary

This study identified differential expression of 190 lncRNAs and 135 mRNAs during intermittent PTH-induced cementogenesis, with eight lncRNAs identified as possible core regulators of the negatively regulated Wnt signaling pathway. The study further verified the repressed expression of key components of the Wnt signaling pathway, suggesting that lncRNAs may play important roles in PTH-induced cementogenesis through Wnt pathway regulation.