期刊
GENOME
卷 64, 期 7, 页码 719-733出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2020-0066
关键词
Aspergillus flavus; genome sequence; secondary metabolism; biosynthetic gene cluster
资金
- National Key R&D Program of China [2018YFC0311001]
- Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD)
- Fundamen tal Research Funds for the Central Universities [SM20190116021]
Marine microorganisms have unique physicochemical characteristics compared to terrestrial microorganisms, producing diverse secondary metabolites (SMs). Three strains of coepiphytic Aspergillus with potential antibacterial activities were isolated from the South China Sea, with A7 showing the highest diversity of biosynthetic gene clusters (BGCs) and superior environmental adaptability. The study provides genetic information for investigating marine Aspergillus spp., potentially elucidating their chemical diversity and adaptive strategies.
Due to the specific properties of the marine environment, marine microorganisms have exclusive physicochemical characteristics that are different from those of terrestrial microorganisms, which can produce various secondary metabolites (SMs) with considerable structural diversity and biological activity. In this study, three strains of coepiphytic Aspergillus with potential antibacterial activities, A7 (Aspergillus flavus), B27 (Aspergillus flavipes), and R12 (Aspergillus sydowii), were isolated from the South China Sea. Via the Illumina MiSeq sequencing platform, the genomes of the three strains were sequenced, and genome comparison showed the highest diversity of the biosynthetic gene clusters (BGCs) in A7. In addition, a comparison of physiological and genomic characteristics between A7 and other A. flavus strains demonstrated the superior environmental adaptability of A7, which is apparently consistent with the genetic richness of BGCs. By assigning reads to known BGCs, putative BGCs were allocated in A7 that corresponded to various SMs, including naphthopyrone, pyranonigrin E, and cyclopiazonic acids. Based on gene homology analysis, we surmise that a region is involved in the biosynthesis of ustiloxin-like RiPPs, a less thoroughly studied SM in fungi. Our results provide genetic information for the investigation of marine Aspergillus spp., which may help to elucidate their chemical diversity and adaptive strategies.
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