期刊
FOOD RESEARCH INTERNATIONAL
卷 139, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2020.109808
关键词
Fermentation; Barley; Identification; UPLC-HRMS; Lactobacillus plantarum dy-1; Metabolomics
资金
- National Natural Science Foundation of China [31701605]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX(20)2036]
- Senior Talent Foundation of Jiangsu University [13JDG038]
Fermentation of barley flour with Lactobacillus plantarum dy-1 led to a gradual slowing down of the process until termination, with decreases in saccharides, amino acids, nucleosides, and some organic acids, and the release of lipids and bioactive molecules. Metabolites were accumulated by L. plantarum dy-1 during fermentation. Characteristic components in the fermented barley aqueous extracts, including functional molecules, were identified through chemometrics analysis, providing insights for further research on functional bioactivities and applications.
Fermentation has been considered as effective tools to promote the functional properties of cereals. In this paper, barley flour was fermented with Lactobacillus plantarum dy-1 (L. plantarum dy-1) and the main components in the fermented barley aqueous extracts were identified using by ultra-high performance liquid chromatography tandem with high resolution mass spectrometry (UPLC-HRMS), and investigated by metabolomics strategy involved on chemometrics. The barley extracts were prepared at the fermentation time of 0, 4, 8, 12, 16, 20, 24, 28 h, respectively and a total of 124 compounds were detected in the samples. Principal component analysis (PCA) was performed and the results indicated that the fermentation process became to slow down from 16 h until terminated. During fermentation, saccharides, amino acids, nucleosides, and some organic acids decreased, while lipids and bioactive molecules in barley were released and metabolites were accumulated by L. plantarum dy-1. Meanwhile, partial least squares discrimination analysis (PLS-DA) was performed for revealing the characteristic components in fermented barley aqueous extracts, including some functional molecules such as indole3-lactic acid, phenyllactic acid, homovanillic acid and cafestol, etc., which provided the roles of them and the basis for further investigation on the functional bioactivities and application.
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