期刊
FOOD CHEMISTRY
卷 334, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.127519
关键词
Broccoli; Genotype; Organs; UHPLC-Triple-TOF-MS; Glucosinolate
资金
- National Key Research and Development Program of China [2017YFD0101805]
- National Nature Science Foundation [31872947, 31501761]
- China Agriculture Research System [CARS-23-A8, CARS-23-E03]
- Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS)
- State Key Laboratory of Vegetable Germplasm Innovation (SKL-VGI), Central Public-interest Scientific Institution Basal Research Fund [Y2020PT35]
In this study, glucosinolate compounds and contents in broccoli were characterized and quantified using UHPLC-Triple-TOF-MS. A total of 80 genotypes and eight developmental organs were analyzed, revealing significant variations in glucosinolate content among different organs, with roots containing the highest amount of glucosinolates.
We aimed to characterize and quantify glucosinolate compounds and contents in broccoli, and a total of 80 genotypes and eight developmental organs were analyzed with UHPLC-Triple-TOF-MS. The method was validated in terms of performance, and the coefficients of determination (R-2) were 0.97 and 0.99 for glucoraphanin and gluconapin, respectively. In 80 genotypes, twelve glucosinolates were found in broccoli florets ranging from 0.467 to 57.156 mu mol/g DW, with the highest glucosinolate content being approximately 122-fold higher than the lowest value. The principal component of glucobrassicin, neoglucobrassicin and glucoraphanin explained 60.53% of the total variance. There were positive correlations among hydroxyglucobrassicin, methoxyglucobrassicin, glucobrassicin, glucoerucin, gluconasturtiin, glucoraphanin, and glucotropaeolin (P < 0.05). The root contained 43% of total glucosinolates in 80 genotypes, and glucoraphanin represented 29% of the total glucosinolate content in different organs. The mutant broccoli genotypes were found by analysis of gluconapin contents in different organs.
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