Sodium 4-styrenesulfonate functionalized nanofibers mat as 96-well plate solid-phase extraction adsorbent for quantitative determination of multiple β-agonists residues in pork samples

In this work, sodium 4-styrenesulfonate functionalized polyacrylonitrile nanofibers mat (SS/PAN NFM) was firstly prepared and applied as 96-well plate solid-phase extraction adsorbent for quantitative determination of seven beta-agonists residues in pork samples. The functional modification endowed the SS/PAN NFM with superior adsorption performance for target beta-agonists. The adsorption process is spontaneous (Delta G < 0), the initial adsorption rate can reach 6.03-9.09 mg/g/min and the maximum adsorption capacity is calculated to be 48.3 mg/g at 298 K. Moreover, SS/PAN NFM can be reused for 12 times without degradation in adsorption capability. Combined with UPLC-MS/MS, the limits of detection can reach 0.006-0.24 mu g/kg, the recoveries ranged from 87.2% to 111% and the relative standard deviations of intra-day and inter-day precisions were in the scope of 1.75%-11.6% and 5.08%-13.5%, respectively. The obtained results fully demonstrated the practicability of this method in preventing the hazard of beta-agonists residues.

Automated summary

In this study, SS/PAN NFM was prepared and applied for solid-phase extraction of seven beta-agonists residues in pork samples. The adsorption process was found to be spontaneous with high initial adsorption rate and maximum capacity. The method showed high sensitivity, good recoveries, and acceptable precision, demonstrating its practicality in preventing the hazard of beta-agonists residues.