期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 147, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2020.111915
关键词
Chaetoglobosin V-b; Anti-inflammation; Antioxidant; Signaling pathway; RAW264.7 cells
资金
- Key Project in Science and Technology Agency of Henan Province [192102110112, 192102110214]
This study found that Cha V-b exerts antioxidant and anti-inflammatory effects by inhibiting key pathways of oxidative stress and inflammation, such as the TLR4-mediated signaling pathway and the TRIF-dependent signaling pathway.
There are few reports on the biological activities of chaetoglobosin V-b (Cha V-b) (a cytochalasin alkaloid). In this study, we investigated the molecular mechanisms underlying the anti-inflammatory and antioxidant effects of Cha V-b in the RAW264.7 cells stimulated lipopolysaccharide (LPS). LPS stimulation-induced oxidative stress (i.e. increase production of reactive oxygen species (ROS) and decreased expression of antioxidant superoxide dismutase (SOD)) was suppressed after a Cha V-b treatment. Cha V-b could significantly inhibit the upregulated expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene and protein induced by LPS whilst attenuating the production of pro-inflammatory cytokines TNF-alpha, IL-6 and IL-1 beta. Such antioxidant and anti-inflammatory effects were achieved through the TLR4-mediated MyD88-dependent signaling pathways (via suppressing the phosphorylation of p38, ERK, JNK MAPK and translocation of the NF-kappa B p65 subunit into nucleus), and the TRIF-dependent signaling pathways (via reducing IFN-beta release without inhibiting interferonregulated factor 3 (IRF3) and IRF7). At 25-100 mu M (a concentration range with no cytotoxicity), Cha V-b dose-dependently influenced SOD enzyme activity and phosphorylation of p38, ERK1/2 and JNK, and at 100 mu M, likely exerted the greatest inhibition towards LPS-induced oxidative stress and inflammatory response via the MAPK and NF-kappa B signaling pathway.
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