Electroencapsulation of Trans-resveratrol in Nanoparticles Composed of Silk Fibroin and Soluble Eggshell Membrane Protein

The beneficial effects of trans-resveratrol on health are widely accepted. However, when exposed to heat and UV light, the degradation of trans-resveratrol to less active form cis-resveratrol limits its use in industrial applications. Due to this reason, it is crucial to preserve the stability of trans-resveratrol by using carrier systems. This study aimed to encapsulate the trans-resveratrol in core/shell nanoparticles composed of eggshell membrane proteins and silk fibroin, respectively, using a coaxial electrospraying technique to preserve its stability. The size of the nanoparticles ranged from 8.2 to 254 nm. Keeping the encapsulation yield at the maximum level (96.9%), electroencapsulation process parameters which minimize the average particle diameter (23.8 nm) were found to be A (silk fibroin concentration) = 30.7 mg/ml, B (ratio of flow rates) = 0.72, C (applied voltage) = 18.8 kV, and D (distance) = 12.2 cm. Encapsulation efficiency varied between 40.05 and 96.41%. Detection of antioxidant capacity of released trans-resveratrol suggested that nanoparticles could be a suitable delivery system for sustained release of trans-resveratrol with preserved thermal and UV photostability. Central composite design (CCD) and the response surface methodology (RSM) were successfully used to optimize the electroencapsulation process parameters for the preparation of trans-resveratrol loaded core/shell nanoparticles. It was found that these parameters seemed to be varied depending on the response required. Therefore, an optimum process should be investigated to obtain desired responses such as high encapsulation yield, high encapsulation efficiency, and small average particle size while preserving the thermal and UV stabilities at reasonable levels.

Automated summary

This study focused on encapsulating trans-resveratrol in core/shell nanoparticles to preserve its stability. Nanoparticles ranging in size from 8.2 to 254 nm were successfully prepared using coaxial electrospraying. The encapsulation process parameters were optimized using Central Composite Design and Response Surface Methodology to achieve high encapsulation yield, efficiency, and small particle size while maintaining thermal and UV stabilities.