期刊
EXPERT REVIEW OF CLINICAL IMMUNOLOGY
卷 17, 期 2, 页码 177-185出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/1744666X.2020.1866987
关键词
Hepatitis B virus; methylation; 5-Aza-2 '-deoxycytidine; t regulatory cells; pd-1; dna methyltransferases
类别
资金
- Ministry of Science and Technology of the People's Republic of China [2017ZX10202202, 2018ZX10302206]
- National Natural Science Foundation of China [81970522]
- Key Research and Development Project of Shandong Province [2019GSF108023]
- China Postdoctoral Science Foundation [2020M672074]
- Shandong University multidisciplinary research and innovation team of young scholars [2020QNQT11]
5-Aza-2´-deoxycytidine treatment increased Tregs and Foxp3 expression in peripheral blood compared to control groups. HBV patients showed a pronounced increase in PD-1 expression, suggesting a potential effect of 5-Aza-2´-deoxycytidine on Treg differentiation in chronic HBV infection.
Objectives: Methylation pattern of gene modification is essential for the differentiation of T regulatory cells (Tregs) and 5-Aza-2MODIFIER LETTER PRIME-deoxycytidine is a common inhibitor of methylation. This study aimed to investigate the potential effects of Treg polarizing conditions and 5-Aza-2MODIFIER LETTER PRIME-deoxycytidine treatment in the differentiation of naive T cells during chronic hepatitis B virus (HBV) infection. Methods: The frequency of Tregs in peripheral blood was determined by flow cytometry from patients with chronic hepatitis B (CHB) (n = 51), liver cirrhosis (LC) (n = 47), hepatocellular carcinoma (HCC) (n = 40) and healthy controls (HCs) (n = 17). Gene expression were detected by qRT-PCR and DNA methyltransferases (DNMT) Activity was also determined. Results: of Tregs and Foxp3 expression in peripheral blood from 5-Aza-2MODIFIER LETTER PRIME-deoxycytidine-treated groups were higher than that with acetic acid treatment as a control. Foxp3 mRNA and the frequency of Tregs derived from naive CD4(+)T cells from peripheral blood of patients with HCC or LC were more pronounced compared with HCs. 5-Aza-2MODIFIER LETTER PRIME-deoxycytidine may have induced a more pronounced upward trend of PD-1 expression in HBV patients. Conclusions: ER LETTER PRIME-deoxycytidine mediated demethylation has potential effects on enhancing the differentiation of naive T cells to Tregs in chronic HBV infection.
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