Comparison of thrombus, gut, and oral microbiomes in Korean patients with ST-elevation myocardial infarction: a case-control study

ST-segment elevation myocardial infarction (STEMI) is characterized by thrombotic coronary artery occlusions caused by atherosclerotic plaque rupture. The gut microbiome potentially contributes to the pathogenesis of coronary artery diseases. This study investigated the microbial diversity and composition of coronary thrombi in STEMI patients and the composition of the thrombus microbiome relative to that of the oral and gut microbiomes. A case-control study was performed with 22 STEMI patients and 20 age- and sex-matched healthy controls. Coronary thrombi were acquired from STEMI patients via manual thrombus aspiration during primary coronary intervention. Oral swab and stool samples were collected from both groups, and 16S rRNA sequencing and metagenomic microbiome analyses were performed. Microbial DNA was detected in 4 of 22 coronary thrombi. Proteobacteria (p) and Bacteroidetes (p) were the most abundant phyla. The oral and gut microbiomes significantly differed between patients and healthy controls. The patient group presented microbial dysbiosis, as follows: a higher relative abundance of Proteobacteria (p) and Enterobacteriaceae (f) in the gut microbiome and a lower abundance of Firmicutes (p) and Haemophilus (g) in the oral microbiome. Furthermore, 4 significantly abundant genera were observed in the coronary thrombus in the patients: Escherichia, 1.25%; Parabacteroides, 0.25%; Christensenella, 0.0%; and Bacteroides, 7.48%. The present results indicate that the relative abundance of the gut and oral microbiomes was correlated with that of the thrombus microbiome. Heart disease: microbiome linked to heart attack Disruption to microbiome composition and functioning may contribute to heart attacks. The most serious form of heart attack is ST-segment elevation myocardial infarction (STEMI), where the coronary artery is blocked by ruptured plaques. Previous research has linked cardiovascular diseases with disruption to the microbiome, but links between STEMI and the microbiome are not yet clear. Si-Hyuck Kang at Seoul National University in Seongnam-si, South Korea, and co-workers analyzed oral, fecal, and coronary thrombus (blood clot) microbial samples from 22 STEMI patients and 20 healthy controls. They found significant differences in oral and gut microbiome composition between the two groups, including increased Proteobacteria phylum and Enterobacteriaceae species and decreased Firmicutes phylum in STEMI patients. Microbes matching patients' oral and gut bacteria were present in four thrombus samples, suggesting that microbes may influence clot formation and plaque rupture.