期刊
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
卷 158, 期 -, 页码 166-171出版社
ELSEVIER
DOI: 10.1016/j.ejpb.2020.11.019
关键词
Bacteriophage therapy; Inhaled phage therapy; Phage and antibiotic combination; Combination therapy; Antibiotic aerosols; Dry powder inhalation (DPI); Mouse lung infection model
资金
- National Health and Medical Research Council [APP1140617]
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R33AI121627]
The study demonstrated that the combination powder of PEV20 and ciprofloxacin significantly reduced the bacterial load in mouse lungs infected with clinical P. aeruginosa strain. The powder also showed reduced lung inflammation associated with its bactericidal effect.
Combination treatment using bacteriophage and antibiotics is potentially an advanced approach to combatting antimicrobial-resistant bacterial infections. We have recently developed an inhalable powder by co-spray drying Pseudomonas phage PEV20 with ciprofloxacin. The purpose of this study was to assess the in vivo effect of the powder using a neutropenic mouse model of acute lung infection. The synergistic activity of PEV20 and ciprofloxacin was investigated by infecting mice with P. aeruginosa, then administering freshly spray-dried single PEV20 (106 PFU/mg), single ciprofloxacin (0.33 mg/mg) or combined PEV20-ciprofloxacin treatment using a dry powder insufflator. Lung tissues were then harvested for colony counting and flow cytometry analysis at 24 h post-treatment. PEV20 and ciprofloxacin combination powder significantly reduced the bacterial load of clinical P. aeruginosa strain in mouse lungs by 5.9 log10 (p < 0.005). No obvious reduction in the bacterial load was observed when the animals were treated only with PEV20 or ciprofloxacin. Assessment of immunological responses in the lungs showed reduced inflammation associating with the bactericidal effect of the PEV20ciprofloxacin powder. In conclusion, this study has demonstrated the synergistic potential of using the combination PEV20-ciprofloxacin powder for P. aeruginosa respiratory infections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据