期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 51, 期 4, 页码 835-847出版社
WILEY
DOI: 10.1002/eji.202048961
关键词
autophagy; cross‐ presentation; DC; LC3; MHCI
类别
资金
- ZonMW TOP [91211011]
This study showed that autophagy negatively affects antigen storage in dendritic cells, and dendritic cells deficient in autophagy exhibited enhanced antigen storage and cross-presentation. The augmented cross-presentation effect was independent of proteasome enzyme activity or MHCI surface expression on dendritic cells. Visualizations indicated that storage compartments were in close proximity to LC3 positive autophagosomes, suggesting that autophagosomes disrupt antigen storage in dendritic cells and regulate long-term MHCI cross-presentation.
Autophagy has been reported to be involved in supporting antigen cross-presentation by dendritic cells (DCs). We have shown that DCs have the ability to store antigen for a prolonged time in endolysosomal compartments and thereby sustain MHCI antigen cross-presentation to CD8(+) T cells. In the current study, we investigated the role of autophagy in long-term antigen presentation. We show that the autophagy machinery has a negative impact on storage of antigen in DCs. Atg5(-/-)DCs which are deficient in autophagy or DCs treated with common autophagy inhibitors showed enhanced antigen storage and antigen cross-presentation. This augmented antigen cross-presentation effect is independent of altered proteasome enzyme activity or MHCI surface expression on DCs. We visualized that the storage compartments are in close proximity to LC3 positive autophagosomes. Our results indicate that autophagosomes disrupt antigen storage in DCs and thereby regulate long-term MHCI cross-presentation.
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