期刊
DEVELOPMENTAL CELL
卷 39, 期 1, 页码 104-115出版社
CELL PRESS
DOI: 10.1016/j.devcel.2016.09.015
关键词
-
资金
- Gates Cambridge Trust
- Sir Henry Wellcome Postdoctoral Fellowship [103060/Z/13/Z]
- MRC DTG [MR/J003808/1]
- Wellcome Trust [WT093736, 095645/Z/11/Z]
- BBSRC
- BBSRC [BB/K010867/1]
- EU BLUEPRINT
- EpiGeneSys
- Swiss National Science Foundation (SNF)/Novartis SNF
- Biotechnology and Biological Sciences Research Council [BBS/E/B/0000H334, BBS/E/B/000C0403, BBS/E/B/0000H331, BBS/E/B/000C0402] Funding Source: researchfish
- Medical Research Council [1520383] Funding Source: researchfish
- Wellcome Trust [103060/Z/13/Z] Funding Source: Wellcome Trust
- BBSRC [BBS/E/B/0000H334, BBS/E/B/0000H331, BBS/E/B/000C0402, BBS/E/B/000C0403] Funding Source: UKRI
Primordial germ cell (PGC) development is characterized by global epigenetic remodeling, which resets genomic potential and establishes an epigenetic ground state. Here we recapitulate PGC specification in vitro from naive embryonic stem cells and characterize the early events of epigenetic reprogramming during the formation of the human and mouse germ line. Following rapid de novo DNA methylation during priming to epiblast-like cells, methylation is globally erased in PGC-like cells. Repressive chromatin marks (H3K9me2/3) and transposable elements are enriched at demethylation-resistant regions, while active chromatin marks (H3K4me3 or H3K27ac) are more prominent at regions that demethylate faster. The dynamics of specification and epigenetic reprogramming show species-specific differences, in particular markedly slower reprogramming kinetics in the human germline. Differences in developmental kinetics may be explained by differential regulation of epigenetic modifiers. Our work establishes a robust and faithful experimental system of the early events of epigenetic reprogramming and regulation in the germline.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据