期刊
DEVELOPMENTAL CELL
卷 37, 期 3, 页码 226-237出版社
CELL PRESS
DOI: 10.1016/j.devcel.2016.04.009
关键词
-
资金
- Global COE Program A06 of Kyoto University
- Naito Foundation, a Sasakawa Scientific Research Grant
- Future Development Funding Program of Kyoto University Research Coordination Alliance [20112006]
- JSPS Research Fellowship
- Minority Postdoctoral Research Fellowship from the NSF [0804021]
- [22124001]
- [17GS0318]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0804021] Funding Source: National Science Foundation
Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon silencing, are also known to act in somatic differentiation of planarian PSCs (neoblasts). However, how PIWIs control the latter process remains elusive. Here, using Dugesia japonica, we show that a nuclear PIWI, DjPiwiB, was bound to PIWI-interacting RNAs (generally key mediators of PIWI-dependent transposon silencing), and was detected in not only neoblasts but also their descendant somatic cells, which do not express piwi. In contrast, cytoplasmic DjPiwiA and DjPiwiC were detected only in neoblasts, in accord with their transcription there. DjPiwiB was indispensable for regeneration, but dispensable for transposon silencing in neoblasts. However, transposons were derepressed at the onset of differentiation in DjPiwiB-knockdown planarians. Thus, DjPiwiB appears to be inherited by descendant somatic cells of neoblasts to ensure transposon silencing in those cells, which are unable to produce PIWI proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据