4.5 Article

In situ H2O2 generation methods in the context of enzyme biocatalysis

期刊

ENZYME AND MICROBIAL TECHNOLOGY
卷 145, 期 -, 页码 -

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2021.109744

关键词

Hydrogen peroxide; Peroxidases; Unspecific peroxygenases; Biocatalysis

资金

  1. U.S. National Science Foundation Emerging Frontiers in Research and Innovation program [1332341]
  2. Emerging Frontiers & Multidisciplinary Activities
  3. Directorate For Engineering [1332341] Funding Source: National Science Foundation

向作者/读者索取更多资源

Hydrogen peroxide is a versatile oxidant used in medical and biotechnology industries, with many enzymes requiring it as a reaction mediator. Generating hydrogen peroxide in situ for biocatalysis has several advantages, including avoiding damage to the catalyst and preventing changes in solution parameters. This synthesis cycle benefits the environment by reducing reliance on nonrenewable chemicals from fossil fuels.
Hydrogen peroxide is a versatile oxidant that has use in medical and biotechnology industries. Many enzymes require this oxidant as a reaction mediator in order to undergo their oxygenation chemistries. While there is a reliable method for generating hydrogen peroxide via an anthraquinone cycle, there are several advantages for generating hydrogen in situ. As highlighted in this review, this is particularly beneficial in the case of biocatalysts that require hydrogen peroxide as a reaction mediator because the exogenous addition of hydrogen peroxide can damage their reactive heme centers and render them inactive. In addition, generation of hydrogen peroxide in situ does not dilute the reaction mixture and cause solution parameters to change. The environment would also benefit from a hydrogen peroxide synthesis cycle that does not rely on nonrenewable chemicals obtained from fossil fuels. Generation of hydrogen peroxide in situ for biocatalysis using enzymes, bioelectrocatalyis, photo catalysis, and cold temperature plasmas are addressed. Particular emphasis is given to reaction processes that support high total turnover numbers (TTNs) of the hydrogen peroxide-requiring enzymes. Discussion of innovations in the use of hydrogen peroxide-producing enzyme cascades for antimicrobial activity, wastewater effluent treatment, and biosensors are also included.

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