4.7 Article

Rhizosphere microbiome dynamics in tropical seagrass under short-term inorganic nitrogen fertilization

期刊

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 28, 期 15, 页码 19021-19033

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s11356-020-12048-5

关键词

Seagrass rhizosphere; Microbiome; Ammonium; Nitrate; Nitrogen addition

资金

  1. Strategic Priority Research Program of the Chinese Academy of Sciences [XDA13020300]
  2. National Natural Science Foundation of China [41676163, 41406191, 41676107, 41976147]
  3. Pearl River S&T Nova Program of Guangzhou [201806010017]
  4. Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0402]
  5. National Key Research and Development Program of China [2018YFC1406505, 2017YFC0506301, 2018FY100105]
  6. Guangdong Province Public Welfare Research and Capacity Building Project [2015A020216016]
  7. Science and Technology Planning Project of Guangdong Province, China [2017B030314052]
  8. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018ZD02]

向作者/读者索取更多资源

This study investigated the impact of inorganic nitrogen on the rhizosphere microbiome of seagrass through high-throughput sequencing, revealing shifts in relative abundance of specific microbial communities under inorganic nitrogen enrichment. Additionally, it was found that seagrass photosynthetic characteristics were influenced by different nitrogen sources.
Rhizosphere microbes are crucial to seagrass meadows because they promote plant growth and heath. However, information concerning the response of rhizosphere microorganisms in seagrass sediment in the presence of different nitrogen sources is lacking. Here, by means of high-throughput sequencing, we investigated how addition of inorganic nitrogen affects the rhizosphere microbiome of the tropical seagrass Thalassia hemperichii. A seagrass culture system was set up to conduct a nitrogen addition (ammonium and nitrate) simulation experiment. We found that the relative abundance of Proteobacteria and Bacteroidetes was increased in inorganic nitrogen-enriched samples, whereas that of Acidobacteria decreased under ammonium enrichment, especially after 35 days. High levels of inorganic nitrogen addition caused a significant decrease in the relative abundance of Desulfobacteraceae, Sulfurovaceae, and Spirochaetes, which are primarily involved in sulfur cycling. Additionally, the abundance of microbes in the seagrass rhizosphere reached the highest after the ammonium-enrichment treatment. Among the analyzed seagrass photosynthetic characteristics, seagrass leaves presented the highest light utility in treatments receiving nitrate, followed by the control groups and ammonium-enrichment groups. Moreover, 16S rRNA gene-predicted functional analysis suggested that some functions related to metabolism of amino acids and signal transduction were enriched in samples receiving high ammonium, whereas nitrate addition enriched predicted functions related to diseases. These findings provide new insights into the response of microbial communities to different types of nitrogen additions in seagrass ecosystems.

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