Antagonistic effect of different selenium type on green synthesized silver nanoparticle toxicity on Oreochromis niloticus: oxidative stress biomarkers

The current investigation pointed to report the negative impacts of silver nanoparticles [Ag](NPs) that synthesized by leaf extract of Moringa oleifera on oxidative stress biomarkers of Oreochromis niloticus, as well as the role of different Selenium type to antagonistic that toxicity. Fish were exposed to [Ag](NPs) (1.95 and 3.9 ppm) as sublethal concentrations alongside 0.1 ppm of Se bulk and 0.1 ppm biosynthesized [Se](NPs) selenium nanoparticles antagonistic effect for 2 and 4 weeks. Lipid peroxidation (LPO), DNA fragmentation, total antioxidant capacity (TAOC), catalase (CAT), and superoxide dismutase (SOD) were evaluated as oxidative stress biomarkers,. A significant increase (p < 0.05) in LPO and DNA fragmentation and a significant decrease (p < 0.05) in TAOC, CAT, and SOD were found in [Ag](NPs) exposed groups when compared with the control one. Biosynthesized [Se](NPs) and Se bulk showed a positive role in [Ag](NPs) detoxification. Our investigation suggested that [Ag](NPs) showed a toxic effect on oxidative stress biomarkers. However, Se-NP addition gives a good recovery of oxidative biomarkers more than Se bulk in detoxification of [Ag](NPs).

Automated summary

The study indicates that silver nanoparticles synthesized by Moringa oleifera leaf extract have negative impacts on oxidative stress biomarkers of Oreochromis niloticus, while selenium nanoparticles can counteract this toxicity. Selenium nanoparticles and selenium bulk show positive roles in detoxification of silver nanoparticles, with selenium nanoparticles having better recovery of oxidative biomarkers compared to selenium bulk.