期刊
DEVELOPMENTAL BIOLOGY
卷 418, 期 2, 页码 297-306出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2016.06.028
关键词
Kidney development; Cap mesenchyme; Nephron progenitor; Cell migration; Live imaging
资金
- Australian Research Council [DE150100652]
- National Health and Medical Research Council of Australia [APP1002748, APP1063696]
- Human Frontiers in Science Program [RGP0039/2011]
- Australian Cancer Research Foundation
- Australian Research Council [DE150100652] Funding Source: Australian Research Council
Morphogenesis of the mammalian kidney requires reciprocal interactions between two cellular domains at the periphery of the developing organ: the tips of the epithelial ureteric tree and adjacent regions of cap mesenchyme. While the presence of the cap mesenchyme is essential for ureteric branching, how it is specifically maintained at the tips is unclear. Using ex vivo timelapse imaging we show that cells of the cap mesenchyme are highly motile. Individual cap mesenchyme cells move within and between cap domains. They also attach and detach from the ureteric tip across time. Timelapse tracks collected for > 800 cells showed evidence that this movement was largely stochastic, with cell autonomous migration influenced by opposing attractive, repulsive and cell adhesion cues. The resulting swarming behaviour maintains a distinct cap mesenchyme domain while facilitating dynamic remodelling in response to underlying changes in the tip. (C) 2016 Elsevier Inc. All rights reserved.
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