期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 62, 期 -, 页码 89-96出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2016.05.002
关键词
Fish; Innate immunity; NF kappa Bi alpha; Gene expression; Genome duplication; Functional annotation
资金
- Modern Agriculture Industry Technology System Construction Projects of China entitled: Staple Freshwater Fishes Industry Technology System [CARS-46-05]
- Fundament Research Funds for the Central Universities [2662015PY019]
- International Scientific and Technology Cooperation Program of Wuhan City [2015030809020365]
I kappa B alpha plays an essential role in the innate immune response in mammals. We found two functional I kappa B alpha paralogs, originating from the teleost-specific genome duplication, in Megalobrama amblycephala: malK B alpha a and maI kappa B alpha b. Their size (936/933 bp) and structure are highly analogous to known orthologs. mRNA expression was analysed by qPCR in spleen, liver, kidney, intestine and gills. Apart from maI kappa B alpha b in gills (<0.001-fold), both paralogs were constitutively expressed in all tissues. Differential expression was observed in gills (high for maI kappa B alpha a) and liver: maI kappa B alpha a - 2nd lowest (0.47), maI kappa B alpha b - 2nd highest (4.25). Both paralogs (mRNA) were upregulated in liver, spleen and kidney after a bacterial (Aeromonas hydrophila) challenge. In spleen, expressions peaked at 12 h post injection (hpi) (maI kappa B alpha a = 14.3-fold, maI kappa B alpha b = 21.3-fold), but only malKBab was highly upregulated at 4, 24 and 120 hpi. In liver, both were upregulated early, but maI kappa B alpha a peaked at 4 hpi (15.2-fold) and maI kappa B alpha b at 12 hpi (9.8-fold). In kidney, maI kappa B alpha a was highly upregulated only at 12 hpi (8.7-fold), and maI kappa B alpha b at 4 (peak - 8.2-fold), 12 and 24 hpi. The results indicate that both I kappa B alpha paralogs have retained their functionality, that they are structurally and functionally homologous to I kappa B alpha orthologs described in other animal species, and that they both play an important role in the innate immune system of M. amblycephala. (C) 2016 Elsevier Ltd. All rights reserved.
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