期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 54, 期 1, 页码 7-19出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.08.007
关键词
Hemipteran innate immunity; Spiroplasma; Immune cells; Hexamerin; Phenoloxidase activity; RNAi
资金
- INRA grant from the department of Plant Health and Environment [SPE 2010_203_0]
Spiroplasma citri is a cell wall-less bacterium that infects plants. It is transmitted by the leafhopper Circulifer haematoceps, which hosts this bacterium in the haemocel and insect tissues. Bacterial factors involved in spiroplasma colonization of the insect host have been identified, but the immune response of the leafhopper to S. citri infection remains unknown. In this study, we showed that C haematoceps activates both humoral and cellular immune responses when challenged with bacteria. When infected by S. citri, C. haematoceps displayed a specific immune response, evidenced by activation of phagocytosis and upregulation of a gene encoding the protein hexamerin. S. citri infection also resulted in decreased phenoloxidase-like activity. Inhibition of hexamerin by RNA interference resulted in a significant reduction in phenoloxidase-like activity and increased mortality of infected leafhoppers. Therefore, the gene hexamerin is involved in S. citri control by interfering with insect phenoloxidase activity. (C) 2015 Elsevier Ltd. All rights reserved.
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