期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 63, 期 -, 页码 96-110出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2016.05.016
关键词
Dendritic cells; Fayoumi; Leghorn; MHC-II; Nitric oxide; Gene expression
资金
- USDA-NIFA-AFRI Climate Change Award [2011-67003-30228]
- USDA National Institute of Food and Agriculture, Hatch project [5358]
- USDA National Needs Fellowship [2011-38420-20050]
- Polish-American Fulbright Commission
Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-gamma, IL-10, IL-12,3, IL-1 beta, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function. (C) 2016 Published by Elsevier Ltd.
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