期刊
DEVELOPMENT GROWTH & DIFFERENTIATION
卷 58, 期 2, 页码 225-237出版社
WILEY-BLACKWELL
DOI: 10.1111/dgd.12268
关键词
adult pluripotent stem cell; chromatoid body; DjCBC-1; PIWI; planarian
资金
- Global COE Program of Kyoto University [A06]
- Naito Foundation, a Sasakawa Scientific Research Grant
- JSPS [13J01064]
- [20112006]
- [22124001]
- [17GS0318]
- Grants-in-Aid for Scientific Research [13J01064] Funding Source: KAKEN
The robust regenerative ability of planarians is known to be dependent on adult pluripotent stem cells called neoblasts. One of the morphological features of neoblasts is cytoplasmic ribonucleoprotein granules (chromatoid bodies: CBs), which resemble germ granules present in germline cells in other animals. Previously, we showed by immuno-electron microscopic analysis that DjCBC-1, a planarian Me31B/Dhh1/DDX6 homologue, which is a component of ribonucleoprotein granules, was localized in CBs in the planarian Dugesia japonica. Also, recently it was reported using another planarian species that Y12 antibody recognizing symmetrical dimethylarginine (sDMA) specifically binds to CBs in which histone mRNA is co-localized. Here, we showed by double immunostaining and RNA interference (RNAi) that DjCBC-1-containing CBs and Y12-immunoreactive CBs are distinct structures, suggesting that CBs are composed of heterogeneous populations. We also found that the Y12-immunoreactive CBs specifically contained a cytoplasmic type of planarian PIWI protein (DjPiwiC). We revealed by RNAi experiments that Y12-immunoreactive CBs may have anti-transposable element activity involving the DjPiwiC protein in the neoblasts.
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