Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment

In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated. In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode. Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control. Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O-H, CH, C = O, C-O, and C-C = O) and assist the detoxification process. Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Q(max)) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively. To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study. Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.

Automated summary

This study investigated the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions and evaluated their metal adsorption efficacy. The results showed that EPS from KMS3-1 strain exhibited efficient adsorption capacity for Cd(II), Cu(II), and Pb(II), making them potential chelating agents for wastewater treatment. The study also confirmed the proficient metal adsorption of EPS through XRD analysis, highlighting their potential application in environmental remediation.