4.7 Article

Chemical forms governing Cd tolerance and detoxification in duckweed (Landoltia punctata)

期刊

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2020.111553

关键词

Subcellular distribution; Chemical forms; Proteins; Cd bioremediation; Landoltia punctata

资金

  1. National Key Research and Development Program of China [2017YFD0800900]
  2. Major Science and Technology Program for Water Pollution Control and Treatment of China [2017ZX07301005-001-01]
  3. Science and Technology Project of Jiangxi Provincial Department of Education, China [GJJ190976]
  4. National Natural Science Foundation of China [51879128, 51568048]

向作者/读者索取更多资源

Duckweed, as an ideal candidate for restoring cadmium-polluted waters, showed that cell wall immobilization and protein binding are primary mechanisms for detoxification of Cd, mainly through changes in chemical forms.
Duckweed (Landoltia punctata) is an ideal species to restore cadmium (Cd)-polluted waters due to its fast growth and easy harvesting. To understand its tolerance and detoxification mechanism, the Cd stress responses, sub -cellular Cd distribution and chemically bound Cd forms (especially protein-bound Cd) were surveyed in this study. L. punctata, a potential Cd bioremediation plant, was cultured hydroponically with Cd concentrations of 0.0, 0.5, 2.0, and 5.0 mg L-1 for 5 days. The results showed that the Cd content in L. punctata increased significantly as the Cd content increased. The majority of Cd was localized in the soluble fraction (23-55%) and the cell wall fraction (21-54%), and only 14-23% of Cd was located in cell organelles. Analysis of the Cd chemical forms demonstrated that the largest portion of Cd was found in 1 M NaCl extracts, followed by d-H2O and 2% HAc extracts, indicating that Cd was mainly bound to different proteins. Albuminand globulin-bound Cd forms were predominant, together accounting for over 80% of the total protein-bound Cd in L. punctata. These results indicate that cell wall immobilization and vacuolar dissociation of Cd are possible primary strategies for Cd biosorption and detoxification in L. punctata, which occur mainly through chemical forms changes, especially the binding of Cd to proteins.

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