期刊
DEVELOPMENT
卷 143, 期 11, 页码 1926-1936出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.136168
关键词
Progesterone; Oocyte maturation; cAMP; Xenopus; PKA
资金
- Qatar National Research Fund (QNRF) [NPRP 7-709-3-195]
- Biomedical Research Program (BMRP) at Weill Cornell Medical College in Qatar - Qatar Foundation
Vertebrate oocytes arrest at prophase of meiosis I as a result of high levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) activity. In Xenopus, progesterone is believed to release meiotic arrest by inhibiting adenylate cyclase, lowering cAMP levels and repressing PKA. However, the exact timing and extent of the cAMP decrease is unclear, with conflicting reports in the literature. Using various in vivo reporters for cAMP and PKA at the single-cell level in real time, we fail to detect any significant changes in cAMP or PKA in response to progesterone. More interestingly, there was no correlation between the levels of PKA inhibition and the release of meiotic arrest. Furthermore, we devised conditions whereby meiotic arrest could be released in the presence of sustained high levels of cAMP. Consistently, lowering endogenous cAMP levels by >65% for prolonged time periods failed to induce spontaneous maturation. These results argue that the release of oocyte meiotic arrest in Xenopus is independent of a reduction in either cAMP levels or PKA activity, but rather proceeds through a parallel cAMP/PKA-independent pathway.
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