期刊
DEVELOPMENTAL CELL
卷 56, 期 1, 页码 141-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.11.013
关键词
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资金
- Wellcome Trust [109081/Z/15/A]
- MRC
- CRUK
- Blood Cancer UK
- NIH-NIDDK
- Sanger-EBI Single Cell Centre
- Wellcome Trust-MRC Cambridge Stem Cell Institute
- Cancer Research UK
- European Molecular Biology Laboratory
- Swedish Research Council [2017-06278]
- Wellcome Strategic award [105031/D/14/Z]
- Wellcome Trust [105031/D/14/Z, 109081/Z/15/A] Funding Source: Wellcome Trust
- MRC [MR/S008799/1, MR/K017047/1, MC_PC_17230] Funding Source: UKRI
- Swedish Research Council [2017-06278] Funding Source: Swedish Research Council
Somite formation is essential for the development of vertebrate body plan, involving three transcriptional trajectories in the early mouse embryo. Anterior somites ingress through the primitive streak before E7, while neuromesodermal progenitors are reserved for later somitogenesis. The role of T in somite development and the potential regulators of early T-independent somites are investigated, challenging the T-Sox2 antagonism model in early NMPs.
Somite formation is foundational to creating the vertebrate segmental body plan. Here, we describe three transcriptional trajectories toward somite formation in the early mouse embryo. Precursors of the anterior-most somites ingress through the primitive streak before E7 and migrate anteriorly by E7.5, while a second wave of more posterior somites develops in the vicinity of the streak. Finally, neuromesodermal progenitors (NMPs) are set aside for subsequent trunk somitogenesis. Single-cell profiling of T-/- chimeric embryos shows that the anterior somites develop in the absence of T and suggests a cell-autonomous function of T as a gatekeeper between paraxial mesoderm production and the building of the NMP pool. Moreover, we identify putative regulators of early T-independent somites and challenge the T-Sox2 cross-antagonism model in early NMPs. Our study highlights the concept of molecular flexibility during early cell-type specification, with broad relevance for pluripotent stem cell differentiation and disease modeling.
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