期刊
DEVELOPMENTAL CELL
卷 55, 期 6, 页码 784-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.11.007
关键词
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资金
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of General Medicine of the National Institutes of Health (NIH) [R01AR068254, R01GM113141]
- National Institute of General Medical Sciences [T32GM08759]
- NCRR [S10RR026680, S10OD021601, S10OD025072]
- NIH [S10OD025267]
Getting large macromolecules through the plasma membrane and endosomal barriers remains a major challenge. Here, we report a generalizable method of delivering proteins and ribonucleoproteins (RNPs) to cells in vitro and mouse liver tissue in vivo with engineered ectosomes. These ectosomes, referred to as Gectosomes,'' are designed to co-encapsulate vesicular stomatitis virus G protein (VSV-G) with bioactive macromolecules via split GFP complementation. We found that this method enables active cargo loading, improves the specific activity of cargo delivery, and facilitates Gectosome purification. Experimental and mathematical modeling analyses suggest that active cargo loading reduces non-specific encapsulation of cellular proteins, particularly nucleic-acid-binding proteins. Using Gectosomes that encapsulate Cre, Ago2, and SaCas9, we demonstrate their ability to execute designed modifications of endogenous genes in cell lines in vitro and mouse liver tissue in vivo, paving the way toward applications of this technology for the treatment of a wide range of human diseases.
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