期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 65, 期 -, 页码 79-88出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2020.06.004
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资金
- Biotechnology and Biological Sciences Research Council [BB/R018189/1]
- European Research Council [339367]
- BBSRC [BB/R018189/1] Funding Source: UKRI
- European Research Council (ERC) [339367] Funding Source: European Research Council (ERC)
The amide bond with its planarity and lack of chemical reactivity is at the heart of protein structure. Chemical methylation of amides is known but was considered too harsh to be accessible to biology. Until last year there was no protein structure in the data bank with an enzymatically methylated amide. The discovery that the natural macrocyclic product, omphalotin is ribosomally synthesized, was not as had been assumed by non-ribosomal peptide synthesis. This was the first definitive evidence that an enzyme could methylate the amide bond. The enzyme, OphMA, iteratively self-hypermethylates its own C-terminus using SAM as cofactor. A second enzyme OphP, a prolyl oligopeptidase cleaves the core peptide from OphMA and cyclizes it into omphalotin. The molecular mechanism for OphMA was elucidated by mutagenesis, structural, biochemical and theoretical studies. This review highlights current progress in peptide N-methylating enzymes.
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