期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 59, 期 -, 页码 193-201出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2020.08.004
关键词
Electrochemically-active bacteria; Geobacter; Microbial fuel cells; Biofilms; Interspecies electron transfer; Microbial nanowires; Pili; Cytochromes; Electron transport; Multimodal imaging; Atomic force microscopy; Cryo-electron microscopy; Protein structure; Conformational change
资金
- Career Award at the Scientific Interfaces from Burroughs Wellcome Fund
- National Institutes of Health [1DP2AI138259-01]
- National Science Foundation (NSF) CAREER award [1749662]
- NSF Early-Concept Grant for Exploratory Research (EAGER) award [2038000]
- Defence Advanced Research Project Agency Army Research Office [W911NF-18-2-0100]
- Charles H. Hood Foundation Child Health Research Award
- Hartwell Foundation Individual Biomedical Research Award
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1749662] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [2038000] Funding Source: National Science Foundation
Extracellular electron transfer via filamentous protein appendages called 'microbial nanowires' has long been studied in Geobacter and other bacteria because of their crucial role in globally-important environmental processes and their applications for bioenergy, biofuels, and bioelectronics. Thousands of papers thought these nanowires as pili without direct evidence. Here, we summarize recent discoveries that could help resolve two decades of confounding observations. Using cryo-electron microscopy with multimodal functional imaging and a suite of electrical, biochemical, and physiological studies, we find that rather than pili, nanowires are composed of cytochromes OmcS and OmcZ that transport electrons via seamless stacking of hemes over micrometers. We discuss the physiological need for two different nanowires and their potential applications for sensing, synthesis, and energy production.
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