期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 59, 期 -, 页码 182-192出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2020.08.005
关键词
Yeast; Saccharomyces cerevisiae; Crabtree-Warburg effect; Metabolic engineering; Pyruvate decarboxylase deficient; Glucose; Ethanol; Acetyl-CoA; Adaptive laboratory evolution
资金
- Great Lakes Bioenergy Research Center, U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0018409]
- Center for Bioenergy and Bioproducts Innovation, U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0018420]
- NIH Biotechnology Training Program Fellowship [NIGMS T32GM135066]
Saccharomyces cerevisiae, Baker's yeast, is the industrial workhorse for producing ethanol and the subject of substantial metabolic engineering research in both industry and academia. S. cerevisiae has been used to demonstrate production of a wide range of chemical products from glucose. However, in many cases, the demonstrations report titers and yields that fall below thresholds for industrial feasibility. Ethanol synthesis is a central part of S. cerevisiae metabolism, and redirecting flux to other products remains a barrier to industrialize strains for producing other molecules. Removing ethanol producing pathways leads to poor fitness, such as impaired growth on glucose. Here, we review metabolic engineering efforts aimed at restoring growth in non-ethanol producing strains with emphasis on relieving glucose repression associated with the Crabtree effect and rewiring metabolism to provide access to critical cellular building blocks. Substantial progress has been made in the past decade, but many opportunities for improvement remain.
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