4.5 Article

Osmotic and ionic regulation, and modulation by protein kinases, FXYD2 peptide and ATP of gill (Na+, K+)-ATPase activity, in the swamp ghost crab Ucides cordatus (Brachyura, Ocypodidae)

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ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2020.110507

关键词

Salinity acclimation; Crab gill microsomal (Na+, K+)-ATPase; FXYD2 peptide; Protein kinases a and C; Ca2+/calmodulin dependent protein kinase; Intracellular signaling cascade

资金

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2013/22625-1, 2016/25336-0]
  2. Conselho de Desenvolvimento Cientifico e Tecnologico [CNPq 445078/2014-6, CNPq 458246/2014-0]
  3. INCT ADAPTA II [465540/2014-7]
  4. Fundacao de Amparo a Pesquisa do Estado do Amazonas [FAPEAM 062.1187/2017]
  5. FAPESP [2013/24252-9]
  6. CNPq [400920/2015-8, 302072/2019-7, 308847/2014-8, 303613/2017-3]

向作者/读者索取更多资源

We analyzed the modulation by exogenous FXYD2 peptide and by endogenous protein kinases A and C, and Ca2+-calmodulin-dependent kinase, of gill (Na+, K+)-ATPase activity in the semi-terrestrial mangrove crab Ucides cordatus after 10-days acclimation to different salinities. Osmotic and ionic regulatory ability and gill (Na-+,Na- K+)-ATPase activity also were evaluated. (Na+, K+)-ATPase activity is stimulated by exogenous pig kidney FXYD2 peptide, while phosphorylation by endogenous protein kinases A and C and Ca2+/calmodulin-dependent kinase inhibits activity. Stimulation by FXYD2 and inhibition by protein kinase C and Ca2+/calmodulin-dependent kinase are salinity-dependent. This is the first demonstration of inhibitory phosphorylation of a crustacean (Na+, K+)-ATPase by Ca2+/calmodulin-dependent kinase. At low salinities, the (Na+, K+)ATPase exhibited a single, low affinity ATP-binding site that showed Michaelis-Menten behavior. Above 18%S, a second, cooperative, high affinity ATP-binding site appeared, corresponding to 10-20% of total (Na+, K+)ATPase activity. Hemolymph osmolality was strongly hyper-/hypo-regulated in crabs acclimated at 2 to 35 parts per thousand S. Cl- was well hyper-/hypo-regulated although Na+ much less so, becoming isonatremic at elevated salinity. (Na+, K+)-ATPase activity was greatest in isosmotic crabs (26 parts per thousand S), decreasing notably at 35 parts per thousand S and also diminishing progressively from 18to 2 parts per thousand S. Hyper-osmoregulation in U. cordatus showed little dependence on gill (Na+, K+)-ATPase activity, suggesting a role for other ion transporters. These findings reveal that the salinity acclimation response in U. cordatus consists of a suite of enzymatic and osmoregulatory adjustments that maintain its osmotic homeostasis in a challenging, mangrove forest environment.

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