期刊
CLINICAL SCIENCE
卷 135, 期 3, 页码 515-534出版社
PORTLAND PRESS LTD
DOI: 10.1042/CS20201122
关键词
-
资金
- Deutsche Forschungsgemeinschaft [AL2054/1-1, VO2259/2-1]
- Else Kroner-Fresenius-Stiftung [2017 A32]
- Berlin Institute of Health (BIH)
- DZHK (German Centre for Cardiovascular Research)
- Bundesministerium fur Bildung und Forschung (BMBF) [FKZ031L0231]
- Charite 3R
The study found that acid sphingomyelinase (ASM)/ceramide is a critical regulator in promoting vascular smooth muscle cell (VSMC) calcification, partly through SGK1-dependent signaling. Inhibiting ASM may help reduce the progression of vascular calcification during chronic kidney disease (CKD).
In chronic kidney disease (CKD), hyperphosphatemia is a key factor promoting medial vascular calcification, a common complication associated with cardiovascular events and high mortality. Vascular calcification involves osteo-/chondrogenic transdifferentiation of vascular smooth muscle cells (VSMCs), but the complex signaling events inducing pro-calcific pathways are incompletely understood. The present study investigated the role of acid sphingomyelinase (ASM)/ceramide as regulator of VSMC calcification. In vitro, both, bacterial sphingomyelinase and phosphate increased ceramide levels in VSMCs. Bacterial sphingomyelinase as well as ceramide supplementation stimulated osteo-/chondrogenic transdifferentiation during control and high phosphate conditions and augmented phosphate-induced calcification of VSMCs. Silencing of serum- and glucocorticoid-inducible kinase 1 (SGK1) blunted the pro-calcific effects of bacterial sphingomyelinase or ceramide. Asm deficiency blunted vascular calcification in a cholecalciferol-overload mouse model and ex vivo isolated-perfused arteries. In addition, Asm deficiency suppressed phosphate-induced osteo-/chondrogenic signaling and calcification of cultured VSMCs. Treatment with the functional ASM inhibitors amitriptyline or fendiline strongly blunted pro-calcific signaling pathways in vitro and in vivo. In conclusion, ASM/ceramide is a critical upstream regulator of vascular calcification, at least partly, through SGK1-dependent signaling. Thus, ASM inhibition by repurposing functional ASM inhibitors to reduce the progression of vascular calcification during CKD warrants further study.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据