Enhancement of polychlorinated biphenyl biodegradation by resuscitation promoting factor (Rpf) and Rpf-responsive bacterial community

The activities of indigenous bacterial communities in polychlorinated biphenyls (PCBs) contaminated environments is closely related to the efficiency of bioremediation processes. Using resuscitation promoting factor (Rpf) from Micrococcus luteus is a promising method for resuscitation and stimulation of functional bacterial populations under stressful conditions. This study aims to use the Rpf to accelerate the biodegradation of Aroclor 1242, and explore putative PCB degraders which were resuscitated by Rpf addition. The Rpf-responsive bacterial populations were investigated using culture-dependent and culture-independent approaches, respectively. The results confirm that Rpf was capable of enhancing PCB degradation of enriched cultures from PCB-contaminated soils, and improving the activities of cultures with low tolerance to PCBs. High-throughput 16S rRNA analysis displays that the Rpf greatly altered the composition and abundance of bacterial populations in the phylum Proteobacteria. Identification of the resuscitated strains further suggests that the Rpf-responsive population was mostly represented by Sphingomonas and Pseudomonas, which are most likely the key PCB-degraders for enhanced biodegradation of PCBs. (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

Using Rpf from Micrococcus luteus can enhance the biodegradation of PCBs in contaminated environments by resuscitating and stimulating functional bacterial populations. It was found that Rpf significantly altered the composition and abundance of bacterial populations in the phylum Proteobacteria, with Sphingomonas and Pseudomonas identified as key PCB-degraders. These results suggest that Rpf has the potential to improve the efficiency of bioremediation processes in PCB-contaminated environments.