期刊
CHEMOSPHERE
卷 263, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2020.128099
关键词
Antibiotic contamination; Vegetable uptake; Soil microorganism; Antibiotic resistance genes
资金
- National Key Research and Development Program of China [2016YFD0800205]
- Research Foundation of BAAFS [QNJJ201829, KJCX20200302]
- National Natural Science Foundation of China [31800098]
- Beijing Natural Science Foundation [5192005, 6182019]
- Beijing Agriculture Innovation Consortium [BAIC04-2020]
- Key Research and Development Program of Ningxia Autonomous Region [2019BFG02015]
This study investigated the distribution of different types of antibiotics in a soil-vegetable system, revealing that both antibiotic type and vegetable species jointly shape the profiles of soil microorganisms and antibiotic resistance genes.
Antibiotic accumulation in soil and plants is an escalating problem in agriculture and is receiving increasing attention. However, the effect of plant species on the fate of different types of antibiotics in a soil-vegetable system and soil resistome has not been adequately explored. To this end, greenhouse pot experiments were conducted to simulate contamination by ciprofloxacin (CIP), oxytetracycline (OTC), sulfamethoxazole (SMZ), and tylosin (TY) at 1 mg kg(-1) in the soils in which cabbage, endive, and spinach were grown. We investigated antibiotic persistence in soils and accumulation in vegetables (i.e., spinach, endive, and cabbage), microbial community profiles, and the abundance of 17 antibiotic resistance genes (ARGs) in contaminated soils. After 40 days, the residues of CIP and OTC in soil and their accumulation in vegetables were significantly higher than those of SMZ and TY. Of all vegetables, spinach had the highest antibiotic accumulation. Further, antibiotic contamination had no significant effect on soil microbial abundance; however, soil microbial diversity significantly decreased in soils amended with TY. The antibiotic type more significantly affected microbial composition than the kind of vegetable species. The relative abundances of some ARGs significantly increased in contaminated soils. Particularly, in endive soil, quinolone-associated cmlA, cmlA2, and qnrS1 increased with CIP contamination, OTC contamination increased tetG2 and otrA, SMZ increased sul1, and TY increased macrolide-related carB and msrc-01 relative abundance. However, some individual ARGs declined upon antibiotic contamination. Our results indicated that antibiotic type and vegetable species jointly shape the profiles of soil microorganisms and ARGs. (C) 2020 Elsevier Ltd. All rights reserved.
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