期刊
CHEMOSPHERE
卷 263, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2020.128020
关键词
Bisphenol A; Circadian clock; Leydig cells; NR1D1; Testosterone
资金
- National Natural Science Foundation of China [31602125, 31771301, 31772817]
- Natural Science Basic Research Plan in Shaanxi Province of China [2019JM-038]
- China Postdoctoral Science Foundation [2017M610658, 2018T111112]
- Shaanxi Postdoctoral Science Foundation [2017BSHEDZZ105]
- Scientific Research Foundation for Talents of Shaanxi [A279021712]
- Scientific Research Foundation for Talents of Northwest AF University [Z11021601]
This study demonstrates that BPA disrupts testosterone production by inhibiting NR1D1 signaling in Leydig cells, leading to a decrease in testosterone levels in mice. Additionally, circadian clock genes are also involved in this process.
Bisphenol A (BPA) is an endocrine-disrupting compound that impairs testosterone synthesis in male mammals. A circadian clock gene deficiency leads to diminished fertility and even infertility in male mice. However, whether circadian clock signaling pathways mediate the suppressive effect of BPA on testosterone synthesis in Leydig cells (LCs) remains unknown. The present study aims to detect the effect of BPA on cellular circadian clock and testosterone synthesis in mouse LCs, and examine the mechanisms underlying NR1D1 signaling. BPA treatment significantly attenuated the transcription levels of Nr1d1 and steroidogenic genes (Hsd3b2 and Hsd17b3) in TM3 cells, but increased other circadian clock gene levels (Per2 and Dbp). BPA treatment also significantly downregulated NR1D1 and StAR protein expression, but upregulated BMAL1 protein expression in TM3 cells. Furthermore, there was a marked decline in testosterone production in BPA-treated TM3 cells. Intraperitoneal injection of BPA profoundly reduced NR1D1 and StAR protein levels and steroidogenic gene transcription levels (Cyp11a1, Hsd3b2, and Hsd17b3), while enhancing BMAL1 protein and other circadian clock gene (Per2 and Dbp) levels in mouse testes. Notably, serum testosterone levels were also drastically reduced in BPA-treated mice. Moreover, SR9009, an NR1D1 agonist, augmented testosterone production in TM3 cells via elevated expression of steroidogenic genes (StAR, Cyp11a1 and Hsd17b3). Conversely, Nr1d1 knockdown inhibited testosterone accumulation and attenuated steroidogenic gene expression. Moreover, treatment with SR9009 partially reversed the BPA effect on the circadian clock and testosterone production. Taken together, our study demonstrates that BPA perturbs testosterone production, at least partially, via inhibiting NR1D1 signaling in LCs. (C) 2020 Elsevier Ltd. All rights reserved.
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