期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 27, 期 2, 页码 766-777出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202003992
关键词
Cells; Europium; Luminescence; Lysosomes; pH
资金
- EPSRC [EP/L01212X/1]
- Cisbio Bioassays
- EPSRC [1930923] Funding Source: UKRI
A set of four luminescent Eu-III complexes with an extended aryl-alkynylpyridine chromophore have been studied, showing significant differences in pH-dependent behavior in their absorption and emission spectral response. The best-behaved probe exhibited no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin, and was able to monitor time-dependent lysosomal acidification effectively. Through optimization of the 'switch-on' intensity ratio, enhancement factors between 250 to 1330 were measured between pH 8 and 4.
A set of four luminescent Eu-III complexes bearing an extended aryl-alkynylpyridine chromophore has been studied, showing very different pH-dependent behaviour in their absorption and emission spectral response. For two complexes with pK(a) values of 6.45 and 6.20 in protein-containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the 'switch-on' intensity ratio could be optimised, and enhancement factors of between 250 to 1330 were measured between pH 8 and 4. The best-behaved probe showed no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin. It was examined in live-cell imaging studies to monitor time-dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was a factor of 50 in NIH-3T3 cells.
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