Selection of a suitable paper membrane for Loop Mediated Isothermal DNA amplification reaction (LAMP) in a point-of-care diagnostic kit-Experimental and CFD analysis

Paper-based point-of-care (POC) devices have attracted tremendous attention because of low-cost, sim-ple fabrication, and portability. LAMP-based POC platform comprises a paper substrate on which the DNA amplification reaction occurs. The quality of the paper substrate plays a critical role in performing an efficient LAMP reaction and for effective visualization of the amplification result. The major objective of the current work is to replace the frequently utilized nitrocellulose (NC) membrane by a cost-effective and high quality paper membrane viz. Whatman filter paper (WF) for rapid detection of E. coli via LAMP reaction. Isolated DNA is added on the paper membranes, which undergo LAMP reaction. The effect of initial DNA concentration and time on LAMP amplification reaction rate is analyzed on paper membranes via image analysis for the first time. Overall study inferred that WF is suitable for effective naked-eye detection and greater extent of LAMP reaction (generation of 19 968 DNA copies). (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

This study focuses on rapid detection of E. coli via LAMP reaction on Whatman filter paper, demonstrating that Whatman filter paper is suitable for effective naked-eye detection and greater extent of LAMP reaction.